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KMID : 0960920030020010001
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Dementia and Neurocognitive Disorders
2003 Volume.2 No. 1 p.1 ~ p.10
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Neuroprotective Effects of Estrogen in SH-SY5Y Human Neuroblastoma Cells
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Yeom Ji-Hyun
Kim Hee
Hong Hyun-Seok
Bang Oh-Young
Huh Kyoon
Mook-In-Hee
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Abstract
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Apoptosis is one major mechanism underlying neuronal cell death in degenerative diseases in the central nervous system. Previous studies have shown that estrogen has neuroprotective effects in several neuronal cell death model systems. In the present study, we established a staurosporine-induced apoptotic neuronal cell death system with human SH-SY5Y cell line. 17bestradiol (E2) blocked staurosporine-induced apoptosis of SH-SY5Y cells, as revealed by MTT as well as LDH assay. The cells showed typical DNA fragmentation at 4 h and 8 h following staurosporine treatment, which was attenuated by E2 pretreatment. Staurosporine-induced chromatin condensation was also reduced by E2 treatment. Because apoptotic stimuli are known to induce caspase-3 activation and PARP cleavage, we examined whether E2 blocks these processes. E2 markedly attenuated staurosporine-induced casapase-3 activation and PARP cleavage. This study shows that E2 blocks staurosporine-induced apoptosis in SH-SY5Y human neuroblastoma cells, suggesting that E2 is a neuroprotective agent that may be used for the treatment of neurodegenerative diseases such as Alzheimer’s disease and Parkinson’s disease.
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KEYWORD
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Alzheimer’s disease, Estrogen, Beta amyloid, Caspase-3, Cell death
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